Once upon a time, mass spectrometers were open-platform devices that could be tweaked as new applications arose. Today’s mass specs, though, are tightly engineered black boxes: sample in, data out. “As the level of sophistication of software and ...
By necessity or convenience, almost everything we know about biochemistry and molecular biology derives from bulk behavior: From gene regulation to Michaelis-Menten kinetics, we understand biology in terms of what the “average” cell in a ...
It's time for a genomics reality check. Despite the constant, glowing coverage of speedy, low-cost next-generation DNA sequencing, whole-genome analysis, and consumer genomics, researchers still have no idea what the vast majority of human genomic DNA ...
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Being able to identify a particular chemical or protein in a piece of tissue isn't always enough; sometimes you need to know exactly how it's distributed there. That question can be answered to some extent by path-lab standbys such as ...
For tandem affinity purification, purified proteins are visualized on a silver-stained gel, then analyzed by liquid chromatographytandem mass spec. Tilmann Buerckstuemmer and Giulio Superti-Furga / Center for Molecular Medicine of the Austrian ...
Epigenetic changes are pivotal events in development and disease. With a constant genome, it is epigenetics - sequence-independent genetic control processes - that exert the molecular forces necessary to help cells remember their molecular heritage, ...
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When it comes to posttranslational modifications, phosphorylation gets most of the attention, but glycosylation is more widespread. Nearly all cell-surface and secreted proteins are adorned with oligosaccharides, which affect cell-cell interactions, ...
Mass spec is a qualitative technique, not a quantitative one. Unless you take some extra steps, the heights of all those mass spectral peaks scattered across the pages of the biological literature do not reflect protein amounts. That's because ...
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